I have a function which, given a NIfTI file, generates a set of images. How can I essentially reverse that process with another function which takes in these images and creates a NIfTI file from them?
How to combine image slices into a NIfTI file
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You do that with SimpleITK's ImageSeriesReader class. It'd look something like this:
Note that the volume won't have any voxel spacing information, so you might need to call the SetSpacing method