I'm getting 403 – Forbidden: Access is denied on IIS v10 ashx files

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I'm getting 403 – Forbidden: Access is denied on IIS v10 when trying to run ashx files.

I'm using classic application pool with identity Local System

Can you please help?

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Deepak-MSFT On BEST ANSWER

I would suggest you check the IIS logs, check the FRT logs, and Check the detailed error message. You could go to IIS > Error pages feature > Edit Features Settings... in Actions pane

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Then, select Detailed errors option and click OK (Refer screenshot):

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For testing purposes, try to give full permission to the site folder.