MuscleCommandLine non-zero return code 1/is not recognized as an internal or external command,

Question

I am trying to align 4 difference sequences using MuscleCommandLine. This code works perfectly on Anaconda and Mac but I am trying to make it work on Windows and I am having several issues.

muscle_exe = r'../muscle3.8.31_i86darwin64.exe'
in_file = r'../infile.fasta'
out_file = r'../aln_out.fasta'
    
muscle_cline = MuscleCommandline(muscle_exe, input=in_file, out=out_file)
muscle_cline()

When I try this using Anaconda on Mac, I get the result. However, when running the same code on Window, I get the following error:

---------------------------------------------------------------------------
ApplicationError                          Traceback (most recent call last)
<ipython-input-3-70ad09443dc6> in <module>
      3 genome = genome_location(before, after, alignment)
      4 
----> 5 filename = custom_fasta(genome, alignment)
      6 
      7 info = AlignIO.read(r'../aln_out.fasta','fasta')

<ipython-input-1-655071c8d454> in custom_fasta(locations_dict, names)
    185 
    186     muscle_cline = MuscleCommandline(muscle_exe, input=in_file, out=out_file)
--> 187     muscle_cline()
    188 
    189     return(filename)

~\Anaconda3\lib\site-packages\Bio\Application\__init__.py in __call__(self, stdin, stdout, stderr, cwd, env)
    567 
    568         if return_code:
--> 569             raise ApplicationError(return_code, str(self), stdout_str, stderr_str)
    570         return stdout_str, stderr_str
    571 

ApplicationError: Non-zero return code 1 from '../muscle3.8.31_i86darwin64.exe -in ../infile.fasta -out ../aln_out.fasta', message "'..' is not recognized as an internal or external command,"

I have read the documentation but this is not listed as an issue. This same question has been asked before but no solution has been offered. People keep asking to install muscle, but the way it works with Biopython and Anaconda is that you do not need to install it.

So why does this keep happening?