Needleman Wunsch with affine gap penalty

Question

Can someone provide an example where an affine gap penalty would eventually give a different result than running NW on two sequences with a fixed gap penalty ?

Answer 1

Affine Gap Penalty Model:

AAAGAATTCA
|||    |||
AAA----TCA

Notice: To get an optimal global alignment, you need to change "End Gap Penalty" to true in "STEP 2 - Set your pairwise alignment options"

Fixed Gap Penalty Model:

AAAGAATTCA
|||   | ||
AAA---T-CA